Isolation and identification of an scFv antibody against nucleocapsid protein of SARS-CoV
Identifieur interne : 003665 ( Main/Exploration ); précédent : 003664; suivant : 003666Isolation and identification of an scFv antibody against nucleocapsid protein of SARS-CoV
Auteurs : Aizhi Zhao [République populaire de Chine] ; Weijun Qin [République populaire de Chine] ; Yueheng Han [République populaire de Chine] ; Weihong Wen [République populaire de Chine] ; Wenhong Zhang [République populaire de Chine] ; Zhonghui Lian [République populaire de Chine] ; Gang Chen [République populaire de Chine] ; Zhuoli Zhang [République populaire de Chine] ; Jianqiang Peng [République populaire de Chine] ; He Wang [République populaire de Chine] ; Yinglu Guo [République populaire de Chine]Source :
- Microbes and Infection [ 1286-4579 ] ; 2007.
Descripteurs français
- KwdFr :
- Analyse de séquence d'ADN, Anticorps antiviraux (immunologie), Banque de peptides, Données de séquences moléculaires, Fragments d'immunoglobuline (), Fragments d'immunoglobuline (génétique), Fragments d'immunoglobuline (immunologie), Humains, Protéines nucléocapside (immunologie), Spécificité des anticorps, Séquence d'acides aminés, Séquence nucléotidique, Virus du SRAS (immunologie).
- MESH :
- génétique : Fragments d'immunoglobuline.
- immunologie : Anticorps antiviraux, Fragments d'immunoglobuline, Protéines nucléocapside, Virus du SRAS.
- Analyse de séquence d'ADN, Banque de peptides, Données de séquences moléculaires, Fragments d'immunoglobuline, Humains, Spécificité des anticorps, Séquence d'acides aminés, Séquence nucléotidique.
English descriptors
- KwdEn :
- Amino Acid Sequence, Antibodies, Viral (immunology), Antibody Specificity, Base Sequence, Humans, Immunoglobulin Fragments (chemistry), Immunoglobulin Fragments (genetics), Immunoglobulin Fragments (immunology), Molecular Sequence Data, Nucleocapsid Proteins (immunology), Peptide Library, SARS Virus (immunology), Sequence Analysis, DNA.
- MESH :
- chemical , chemistry : Immunoglobulin Fragments.
- chemical , genetics : Immunoglobulin Fragments.
- chemical , immunology : Antibodies, Viral, Immunoglobulin Fragments, Nucleocapsid Proteins.
- immunology : SARS Virus.
- Amino Acid Sequence, Antibody Specificity, Base Sequence, Humans, Molecular Sequence Data, Peptide Library, Sequence Analysis, DNA.
Abstract
To develop reagents for early diagnosis and therapeutic drugs against SARS-associated coronavirus (SARS-CoV), a large (3 × 109) immunized human antibody library was constructed from peripheral blood mononuclear cells from six SARS convalescent patients. A single chain variable fragment antibody (N18) with high affinity against N protein of SARS-CoV was isolated. Sequence analysis revealed that the
Url:
DOI: 10.1016/j.micinf.2007.04.008
PubMed: 17548223
PubMed Central: 7110486
Affiliations:
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Le document en format XML
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Gang" sort="Chen, Gang" uniqKey="Chen G" first="Gang" last="Chen">Gang Chen</name><affiliation wicri:level="1"><nlm:aff id="aff3">Department of Biochemistry and Biology, The Fourth Military Medical University, Xi'an 710032, People's Republic of China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Biochemistry and Biology, The Fourth Military Medical University, Xi'an 710032</wicri:regionArea><wicri:noRegion>Xi'an 710032</wicri:noRegion></affiliation></author><author><name sortKey="Zhang, Zhuoli" sort="Zhang, Zhuoli" uniqKey="Zhang Z" first="Zhuoli" last="Zhang">Zhuoli Zhang</name><affiliation wicri:level="1"><nlm:aff id="aff3">Department of Biochemistry and Biology, The Fourth Military Medical University, Xi'an 710032, People's Republic of China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Biochemistry and Biology, The Fourth Military Medical University, Xi'an 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xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Vector Gene Technology Company Ltd., Beijing 100176</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Qin, Weijun" sort="Qin, Weijun" uniqKey="Qin W" first="Weijun" last="Qin">Weijun Qin</name><affiliation wicri:level="1"><nlm:aff id="aff2">Department of Urology, Xijing Hospital, The Fourth Military Medical University, Xi'an 710032, People's Republic of China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Urology, Xijing Hospital, The Fourth Military Medical University, Xi'an 710032</wicri:regionArea><wicri:noRegion>Xi'an 710032</wicri:noRegion></affiliation><affiliation wicri:level="4"><nlm:aff id="aff4">Institute of Urology, The First Hospital, Peking University, Beijing 100034, People's Republic of China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Urology, The First Hospital, Peking University, Beijing 100034</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName><orgName type="university">Université de Pékin</orgName><placeName><settlement type="city">Pékin</settlement><region type="capitale">Pékin</region></placeName></affiliation></author><author><name sortKey="Han, Yueheng" sort="Han, Yueheng" uniqKey="Han Y" first="Yueheng" last="Han">Yueheng Han</name><affiliation wicri:level="1"><nlm:aff id="aff3">Department of Biochemistry and Biology, The Fourth Military Medical University, Xi'an 710032, People's Republic of China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Biochemistry and Biology, The Fourth Military Medical University, Xi'an 710032</wicri:regionArea><wicri:noRegion>Xi'an 710032</wicri:noRegion></affiliation></author><author><name sortKey="Wen, Weihong" sort="Wen, Weihong" uniqKey="Wen W" first="Weihong" last="Wen">Weihong Wen</name><affiliation wicri:level="1"><nlm:aff id="aff3">Department of Biochemistry and Biology, The Fourth Military Medical University, Xi'an 710032, People's Republic of China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Biochemistry and Biology, The Fourth Military Medical University, Xi'an 710032</wicri:regionArea><wicri:noRegion>Xi'an 710032</wicri:noRegion></affiliation></author><author><name sortKey="Zhang, Wenhong" sort="Zhang, Wenhong" uniqKey="Zhang W" first="Wenhong" last="Zhang">Wenhong Zhang</name><affiliation wicri:level="1"><nlm:aff id="aff3">Department of Biochemistry and Biology, The Fourth Military Medical University, Xi'an 710032, People's Republic of China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Biochemistry and Biology, The Fourth Military Medical University, Xi'an 710032</wicri:regionArea><wicri:noRegion>Xi'an 710032</wicri:noRegion></affiliation></author><author><name sortKey="Lian, Zhonghui" sort="Lian, Zhonghui" uniqKey="Lian Z" first="Zhonghui" last="Lian">Zhonghui Lian</name><affiliation wicri:level="1"><nlm:aff id="aff1">Vector Gene Technology Company Ltd., Beijing 100176, People's Republic of China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Vector Gene Technology Company Ltd., Beijing 100176</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Chen, Gang" sort="Chen, Gang" uniqKey="Chen G" first="Gang" last="Chen">Gang Chen</name><affiliation wicri:level="1"><nlm:aff id="aff3">Department of Biochemistry and Biology, The Fourth Military Medical University, Xi'an 710032, People's Republic of China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Biochemistry and Biology, The Fourth Military Medical University, Xi'an 710032</wicri:regionArea><wicri:noRegion>Xi'an 710032</wicri:noRegion></affiliation></author><author><name sortKey="Zhang, Zhuoli" sort="Zhang, Zhuoli" uniqKey="Zhang Z" first="Zhuoli" last="Zhang">Zhuoli Zhang</name><affiliation wicri:level="1"><nlm:aff id="aff3">Department of Biochemistry and Biology, The Fourth Military Medical University, Xi'an 710032, People's Republic of China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Biochemistry and Biology, The Fourth Military Medical University, Xi'an 710032</wicri:regionArea><wicri:noRegion>Xi'an 710032</wicri:noRegion></affiliation></author><author><name sortKey="Peng, Jianqiang" sort="Peng, Jianqiang" uniqKey="Peng J" first="Jianqiang" last="Peng">Jianqiang Peng</name><affiliation wicri:level="1"><nlm:aff id="aff1">Vector Gene Technology Company Ltd., Beijing 100176, People's Republic of China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Vector Gene Technology Company Ltd., Beijing 100176</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Wang, He" sort="Wang, He" uniqKey="Wang H" first="He" last="Wang">He Wang</name><affiliation wicri:level="1"><nlm:aff id="aff2">Department of Urology, Xijing Hospital, The Fourth Military Medical University, Xi'an 710032, People's Republic of China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Urology, Xijing Hospital, The Fourth Military Medical University, Xi'an 710032</wicri:regionArea><wicri:noRegion>Xi'an 710032</wicri:noRegion></affiliation></author><author><name sortKey="Guo, Yinglu" sort="Guo, Yinglu" uniqKey="Guo Y" first="Yinglu" last="Guo">Yinglu Guo</name><affiliation wicri:level="4"><nlm:aff id="aff4">Institute of Urology, The First Hospital, Peking University, Beijing 100034, People's Republic of China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Urology, The First Hospital, Peking University, Beijing 100034</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName><orgName type="university">Université de Pékin</orgName><placeName><settlement type="city">Pékin</settlement><region type="capitale">Pékin</region></placeName></affiliation></author></analytic><series><title level="j">Microbes and Infection</title><idno type="ISSN">1286-4579</idno><idno type="eISSN">1769-714X</idno><imprint><date when="2007">2007</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Antibodies, Viral (immunology)</term><term>Antibody Specificity</term><term>Base Sequence</term><term>Humans</term><term>Immunoglobulin Fragments (chemistry)</term><term>Immunoglobulin Fragments (genetics)</term><term>Immunoglobulin Fragments (immunology)</term><term>Molecular Sequence Data</term><term>Nucleocapsid Proteins (immunology)</term><term>Peptide Library</term><term>SARS Virus (immunology)</term><term>Sequence Analysis, DNA</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Analyse de séquence d'ADN</term><term>Anticorps antiviraux (immunologie)</term><term>Banque de peptides</term><term>Données de séquences moléculaires</term><term>Fragments d'immunoglobuline ()</term><term>Fragments d'immunoglobuline (génétique)</term><term>Fragments d'immunoglobuline (immunologie)</term><term>Humains</term><term>Protéines nucléocapside (immunologie)</term><term>Spécificité des anticorps</term><term>Séquence d'acides aminés</term><term>Séquence nucléotidique</term><term>Virus du SRAS (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Immunoglobulin Fragments</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Immunoglobulin Fragments</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Viral</term><term>Immunoglobulin Fragments</term><term>Nucleocapsid Proteins</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Fragments d'immunoglobuline</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps antiviraux</term><term>Fragments d'immunoglobuline</term><term>Protéines nucléocapside</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Antibody Specificity</term><term>Base Sequence</term><term>Humans</term><term>Molecular Sequence Data</term><term>Peptide Library</term><term>Sequence Analysis, DNA</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de séquence d'ADN</term><term>Banque de peptides</term><term>Données de séquences moléculaires</term><term>Fragments d'immunoglobuline</term><term>Humains</term><term>Spécificité des anticorps</term><term>Séquence d'acides aminés</term><term>Séquence nucléotidique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>To develop reagents for early diagnosis and therapeutic drugs against SARS-associated coronavirus (SARS-CoV), a large (3 × 10<sup>9</sup>) immunized human antibody library was constructed from peripheral blood mononuclear cells from six SARS convalescent patients. A single chain variable fragment antibody (N18) with high affinity against N protein of SARS-CoV was isolated. Sequence analysis revealed that the <italic>VL</italic> gene was composed of VL3 h (V lambda subgroup) and JL2 regions and the <italic>VH</italic> gene was composed of VH1-69 (VH1 subgroup), D2-15, D3-22 and JH6 regions. Soluble N18 antibody was expressed in <italic>Escherichia coli</italic> HB2151, purified by Ni–NTA affinity chromatography and verified by SDS-PAGE and Western blot. The potential application for early diagnosis was evaluated using N protein capture ELISA in which N18 antibody demonstrated high sensitive activity in detecting N protein of SARS-CoV. Finally, the potential usefulness of the N18 antibody in prophylaxis, vaccine design and therapy of SARS is discussed.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Li, Y H" uniqKey="Li Y">Y.H. 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Greenberg</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Pékin</li></region><settlement><li>Pékin</li></settlement><orgName><li>Université de Pékin</li></orgName></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Zhao, Aizhi" sort="Zhao, Aizhi" uniqKey="Zhao A" first="Aizhi" last="Zhao">Aizhi Zhao</name></noRegion><name sortKey="Chen, Gang" sort="Chen, Gang" uniqKey="Chen G" first="Gang" last="Chen">Gang Chen</name><name sortKey="Guo, Yinglu" sort="Guo, Yinglu" uniqKey="Guo Y" first="Yinglu" last="Guo">Yinglu Guo</name><name sortKey="Han, Yueheng" sort="Han, Yueheng" uniqKey="Han Y" first="Yueheng" last="Han">Yueheng Han</name><name sortKey="Lian, Zhonghui" sort="Lian, Zhonghui" uniqKey="Lian Z" first="Zhonghui" last="Lian">Zhonghui Lian</name><name sortKey="Peng, Jianqiang" sort="Peng, Jianqiang" uniqKey="Peng J" first="Jianqiang" last="Peng">Jianqiang Peng</name><name sortKey="Qin, Weijun" sort="Qin, Weijun" uniqKey="Qin W" first="Weijun" last="Qin">Weijun Qin</name><name sortKey="Qin, Weijun" sort="Qin, Weijun" uniqKey="Qin W" first="Weijun" last="Qin">Weijun Qin</name><name sortKey="Wang, He" sort="Wang, He" uniqKey="Wang H" first="He" last="Wang">He Wang</name><name sortKey="Wen, Weihong" sort="Wen, Weihong" uniqKey="Wen W" first="Weihong" last="Wen">Weihong Wen</name><name sortKey="Zhang, Wenhong" sort="Zhang, Wenhong" uniqKey="Zhang W" first="Wenhong" last="Zhang">Wenhong Zhang</name><name sortKey="Zhang, Zhuoli" sort="Zhang, Zhuoli" uniqKey="Zhang Z" first="Zhuoli" last="Zhang">Zhuoli Zhang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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